A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.
Identifieur interne : 000C93 ( Main/Exploration ); précédent : 000C92; suivant : 000C94A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.
Auteurs : Dujuan Li [République populaire de Chine] ; Jianping Wang ; Ronghui Wang ; Yanbin Li ; Daad Abi-Ghanem ; Luc Berghman ; Billy Hargis ; Huaguang LuSource :
- Biosensors & bioelectronics [ 1873-4235 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux, Anticorps immobilisés, Humains, Microscopie électronique à balayage, Nanoparticules de magnétite (ultrastructure), Poulets, Sous-type H5N1 du virus de la grippe A (immunologie), Sous-type H5N1 du virus de la grippe A (isolement et purification), Sous-type H5N1 du virus de la grippe A (ultrastructure), Techniques de biocapteur (instrumentation), Techniques de microbalance à cristal de quartz (instrumentation), Virologie (instrumentation).
- MESH :
- immunologie : Sous-type H5N1 du virus de la grippe A.
- isolement et purification : Sous-type H5N1 du virus de la grippe A.
- ultrastructure : Animaux, Anticorps antiviraux, Anticorps immobilisés, Humains, Microscopie électronique à balayage, Nanoparticules de magnétite, Poulets, Sous-type H5N1 du virus de la grippe A, Techniques de biocapteur, Techniques de microbalance à cristal de quartz, Virologie.
English descriptors
- KwdEn :
- Animals, Antibodies, Immobilized, Antibodies, Viral, Biosensing Techniques (instrumentation), Chickens, Humans, Influenza A Virus, H5N1 Subtype (immunology), Influenza A Virus, H5N1 Subtype (isolation & purification), Influenza A Virus, H5N1 Subtype (ultrastructure), Magnetite Nanoparticles (ultrastructure), Microscopy, Electron, Scanning, Quartz Crystal Microbalance Techniques (instrumentation), Virology (instrumentation).
- MESH :
- chemical , ultrastructure : Magnetite Nanoparticles.
- chemical : Antibodies, Immobilized, Antibodies, Viral.
- immunology : Influenza A Virus, H5N1 Subtype.
- instrumentation : Biosensing Techniques, Quartz Crystal Microbalance Techniques, Virology.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- ultrastructure : Influenza A Virus, H5N1 Subtype.
- Animals, Chickens, Humans, Microscopy, Electron, Scanning.
Abstract
As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.
DOI: 10.1016/j.bios.2011.04.010
PubMed: 21536419
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000173
- to stream PubMed, to step Curation: 000173
- to stream PubMed, to step Checkpoint: 000166
- to stream Ncbi, to step Merge: 000644
- to stream Ncbi, to step Curation: 000644
- to stream Ncbi, to step Checkpoint: 000644
- to stream Main, to step Merge: 000C98
- to stream Main, to step Curation: 000C93
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.</title><author><name sortKey="Li, Dujuan" sort="Li, Dujuan" uniqKey="Li D" first="Dujuan" last="Li">Dujuan Li</name><affiliation wicri:level="4"><nlm:affiliation>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wang, Jianping" sort="Wang, Jianping" uniqKey="Wang J" first="Jianping" last="Wang">Jianping Wang</name></author><author><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></author><author><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name></author><author><name sortKey="Abi Ghanem, Daad" sort="Abi Ghanem, Daad" uniqKey="Abi Ghanem D" first="Daad" last="Abi-Ghanem">Daad Abi-Ghanem</name></author><author><name sortKey="Berghman, Luc" sort="Berghman, Luc" uniqKey="Berghman L" first="Luc" last="Berghman">Luc Berghman</name></author><author><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name></author><author><name sortKey="Lu, Huaguang" sort="Lu, Huaguang" uniqKey="Lu H" first="Huaguang" last="Lu">Huaguang Lu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21536419</idno><idno type="pmid">21536419</idno><idno type="doi">10.1016/j.bios.2011.04.010</idno><idno type="wicri:Area/PubMed/Corpus">000173</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000173</idno><idno type="wicri:Area/PubMed/Curation">000173</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000173</idno><idno type="wicri:Area/PubMed/Checkpoint">000166</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000166</idno><idno type="wicri:Area/Ncbi/Merge">000644</idno><idno type="wicri:Area/Ncbi/Curation">000644</idno><idno type="wicri:Area/Ncbi/Checkpoint">000644</idno><idno type="wicri:Area/Main/Merge">000C98</idno><idno type="wicri:Area/Main/Curation">000C93</idno><idno type="wicri:Area/Main/Exploration">000C93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.</title><author><name sortKey="Li, Dujuan" sort="Li, Dujuan" uniqKey="Li D" first="Dujuan" last="Li">Dujuan Li</name><affiliation wicri:level="4"><nlm:affiliation>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wang, Jianping" sort="Wang, Jianping" uniqKey="Wang J" first="Jianping" last="Wang">Jianping Wang</name></author><author><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></author><author><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name></author><author><name sortKey="Abi Ghanem, Daad" sort="Abi Ghanem, Daad" uniqKey="Abi Ghanem D" first="Daad" last="Abi-Ghanem">Daad Abi-Ghanem</name></author><author><name sortKey="Berghman, Luc" sort="Berghman, Luc" uniqKey="Berghman L" first="Luc" last="Berghman">Luc Berghman</name></author><author><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name></author><author><name sortKey="Lu, Huaguang" sort="Lu, Huaguang" uniqKey="Lu H" first="Huaguang" last="Lu">Huaguang Lu</name></author></analytic><series><title level="j">Biosensors & bioelectronics</title><idno type="eISSN">1873-4235</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Immobilized</term><term>Antibodies, Viral</term><term>Biosensing Techniques (instrumentation)</term><term>Chickens</term><term>Humans</term><term>Influenza A Virus, H5N1 Subtype (immunology)</term><term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term><term>Influenza A Virus, H5N1 Subtype (ultrastructure)</term><term>Magnetite Nanoparticles (ultrastructure)</term><term>Microscopy, Electron, Scanning</term><term>Quartz Crystal Microbalance Techniques (instrumentation)</term><term>Virology (instrumentation)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux</term><term>Anticorps immobilisés</term><term>Humains</term><term>Microscopie électronique à balayage</term><term>Nanoparticules de magnétite (ultrastructure)</term><term>Poulets</term><term>Sous-type H5N1 du virus de la grippe A (immunologie)</term><term>Sous-type H5N1 du virus de la grippe A (isolement et purification)</term><term>Sous-type H5N1 du virus de la grippe A (ultrastructure)</term><term>Techniques de biocapteur (instrumentation)</term><term>Techniques de microbalance à cristal de quartz (instrumentation)</term><term>Virologie (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Magnetite Nanoparticles</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Immobilized</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>Quartz Crystal Microbalance Techniques</term><term>Virology</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Humans</term><term>Microscopy, Electron, Scanning</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux</term><term>Anticorps immobilisés</term><term>Humains</term><term>Microscopie électronique à balayage</term><term>Nanoparticules de magnétite</term><term>Poulets</term><term>Sous-type H5N1 du virus de la grippe A</term><term>Techniques de biocapteur</term><term>Techniques de microbalance à cristal de quartz</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as a new method and application for AI H5N1 virus detection. Polyclonal antibodies against AI H5N1 virus surface antigen HA (Hemagglutinin) were immobilized on the gold surface of the QCM crystal through self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA). Target H5N1 viruses were then captured by the immobilized antibodies, resulting in a change in the frequency. Magnetic nanobeads (diameter, 30nm) coated with anti-H5 antibodies were used for further amplification of the binding reaction between antibody and antigen (virus). Both bindings of target H5N1 viruses and magnetic nanobeads onto the crystal surface were further confirmed by environmental scanning electron microscopy (ESEM). The QCM immunosensor could detect the H5N1 virus at a titer higher than 0.0128 HA unit within 2h. The nanobeads amplification resulted in much better detection signal for target virus with lower titers. The response of the antibody-antigen (virus) interaction was shown to be virus titer-dependent, and a linear correlation between the logarithmic number of H5N1 virus titers and frequency shift was found from 0.128 to 12.8 HA unit. No significant interference was observed from non-target subtypes such as AI subtypes H3N2, H2N2, and H4N8. The immunosensor was evaluated using chicken tracheal swab samples. This research demonstrated that the magnetic nanobeads amplification based QCM immunosensor has a great potential to be an alternative method for rapid, sensitive, and specific detection of AI virus H5N1 in agricultural, food, environmental and clinical samples.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Abi Ghanem, Daad" sort="Abi Ghanem, Daad" uniqKey="Abi Ghanem D" first="Daad" last="Abi-Ghanem">Daad Abi-Ghanem</name><name sortKey="Berghman, Luc" sort="Berghman, Luc" uniqKey="Berghman L" first="Luc" last="Berghman">Luc Berghman</name><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name><name sortKey="Lu, Huaguang" sort="Lu, Huaguang" uniqKey="Lu H" first="Huaguang" last="Lu">Huaguang Lu</name><name sortKey="Wang, Jianping" sort="Wang, Jianping" uniqKey="Wang J" first="Jianping" last="Wang">Jianping Wang</name><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Li, Dujuan" sort="Li, Dujuan" uniqKey="Li D" first="Dujuan" last="Li">Dujuan Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000C93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21536419
|texte= A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21536419" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |